Isolation and characterization of strains of the Bacillus associated to rice (Oryza sativa L.) crop

Authors

  • Marcia María Rojas Badía Facultad de Biología, Universidad de la Habana, Habana-Cuba
  • Berto Tejera Hernández Facultad de Biología Universidad de La Habana
  • Jeny A. Larrea Murrel Facultad de Biología Universidad de La Habana
  • Jacques Mahillon Université Catholique de Louvain, Louvain-la-Neuve- Belgium
  • Mayra Heydrich Pérez Facultad de Biología Universidad de La Habana

DOI:

https://doi.org/10.33240/rba.v6i1.49175

Keywords:

Bacillus, rice, biological control,, plant growth promotion

Abstract

This work shows the isolation and characterization of Bacillus strains from rhizosphere of rice crop (Oryza sativa L.). Two isolation methods were used, microcosm model to isolates bacteria from variety J-104 and direct isolation from soil cultivated with INCA LP-5 variety. Physiological characterization of 13 isolates was carried out, considering indole compounds production, phosphate solubilization, qualitative nitrogen fixing ability and antagonistic effect against phytophogenic fungi of rice (Alternaria solani, Pyricularia grisea, Fusarium sp. and Curvularia sp.). A conglomerated cluster analysis was performed to select the best isolates to be identified by biochemical test and 16S rDNA sequencing

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Published

2023-06-17

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Section

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