Bases para o Desenvolvimento de uma Plataforma Organ-on-a-chip: Engenharia Biomédica no CHIP-eny Nacional
Abstract
Diabetic foot is a severe complication of Diabetes Mellitus, resulting from peripheral neuropathy, peripheral arterial disease, and infections, and may progress to ulcers and amputations. Conventional treatment is often ineffective in advanced stages, highlighting the need for new technologies for both study and intervention. In response to this challenge, researchers at the University of Brasília developed the ENY chip, a microfluidic device based on Organ-on-a-Chip (OoC) technology, capable of simulating the microenvironment of diabetic wounds for pharmacological testing and the evaluation of assistive medical devices.
To accelerate the development of the chip, structural and fluid dynamic computational simulations were performed. The fluid dynamics simulations, conducted under steady-state conditions with incompressible flow and adiabatic walls, used saline solution as the working fluid. The fluid properties were analyzed at three temperatures (35°C, 37°C, and 40°C), covering kinematic viscosity, dynamic viscosity, and density. The study revealed a laminar profile with maximum velocity at the center of the microchannels and a smooth gradient near the walls, reflecting a typical biological flow behavior.
The pressure distribution showed a progressive drop between inlet and outlet, with a peak at the inlet due to the peristaltic pump. The shear stress analysis identified critical areas at the edges of the microchannels—relevant for predicting structural wear—but all values remained within safe thresholds. Structural simulations validated the chip’s resistance to expected mechanical stresses, ensuring stability and safety for cell culture.
In addition to optimizing the design, the simulations reduced development costs and accelerated the innovation cycle by enabling adjustments prior to physical prototyping and the transition to experimental testing. This study highlights the impact of computational simulations on biomedical device innovation, driving progress in the treatment of diabetic wounds and the development of clinically relevant assistive technologies.
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